Screening for the Detection of IgG anti-Toxoplasma gondii Antibodies in Female Donors at Semarang Regency
Abstract
Toxoplasmosis is a widespread zoonotic disease caused by Toxoplasma gondii (T.gondii). Blood and blood products are possible routes of transmission of T. gondii, especially in patients who have undergone multiple transfusions. The aim of this study is to determine the seroprevalence of toxoplasmosis in female donors using blood serum which is analyzed serologically to detect ImmunoglobulinG (IgG) using the Enzyme-Linked Fluorescent Assay (ELFA) method, as well as the relationship between risk factors for toxoplasmosis, such as keeping cats, receiving blood transfusions, eating undercooked meat and eating raw vegetables. This research is an analytic observational study using a cross-sectional study design. The sample taken is female donor candidates who are willing to participate during the study period, from March to September 2023, and have met the inclusion criteria to become research subjects by explaining the purpose and procedure of the study and the consent letter signed by each research subject before blood samples were taken and filling out the research questionnaire. 177 blood serum samples of female donors were collected from blood donor candidates using consecutive sampling method and a questionnaire to obtain risk factor data that influence the incidence of toxoplasmosis. The results showed that the seroprevalence of toxoplasmosis in the blood of female donor at Indonesian Red Cross Blood Donor Unit (UDD PMI) Semarang Regency, Central Java Province was 26.6%. In this study the risk factors that influence the incidence of toxoplasmosis are consuming raw vegetables with 5.835 times more risk than women who do not consume raw vegetables, while other factors such as keeping cats, receiving blood transfusions, and eating undercooked meat, have no significant effect. The seroprevalence of toxoplasmosis in female donors at UDD PMI of Semarang Regency is quite high due to the habit of eating raw vegetables or fresh vegetables with a very high transmission potential.
How to Cite
Views / Downloads
Files
License
Authors who publish with Poltekita : Jurnal Ilmu Kesehatan agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License (CC BY-SA 4.0) that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.
This work is licensed under a Creative Commons Attribution-ShareAlike 2.0 Generic License.
Poltekita : Jurnal Ilmu Kesehatan is licensed under a Creative Commons Attribution-Share Alike 4.0 International License
You are free to:
- Share, copy and redistribute the material in any medium or format
- Adapt, remix, transform, and build upon the material for any purpose, even commercially.
- The licensor cannot revoke these freedoms as long as you follow the license terms.
References
Rahimi MT, Daryani A, Sarvi S, Shokri A, Ahmadpour E, Teshnizi SH, et al. Cats and Toxoplasma gondii: A systematic review and meta-analysis in Iran. Onderstepoort Journal of Veterinary Research. 2015;82(1).
Naeini KM, Soureshjani EH, Jafari M, Parchami S, Karimi G, Abdizadeh R. Prevalence of Toxoplasma gondii infection in healthy volunteer blood donors using serological and molecular methods from chaharmahal and Bakhtiari Province, Southwest Iran. Jundishapur J Microbiol. 2019;12(5).
Daryani A, Sarvi S, Aarabi M, Mizani A, Ahmadpour E, Shokri A, et al. Seroprevalence of Toxoplasma gondii in the Iranian general population: A systematic review and meta-analysis. Acta tropica, 2014; 137, 185-194.
Foroutan-Rad M, Majidiani H, Dalvand S, Daryani A, Kooti W, Saki J, et al. Toxoplasmosis in Blood Donors: A Systematic Review and Meta-Analysis. Transfusion medicine reviews, 2016; 30(3), 116-122.
Stephen S, Pradeep J, Anitharaj V, Janarthanam V. Seroprevalence of toxoplasmosis in voluntary blood donors of Puducherry and surrounding districts of Tamil Nadu. Journal of Parasitic Diseases. 2017 Dec 1;41(4):1158–61.
Belluco S, Mancin M, Conficoni D, Simonato G, Pietrobelli M, Ricci A. Investigating the determinants of Toxoplasma gondii prevalence in meat: A systematic review and meta-regression. PLoS One. 2016 Apr 1;11(4).
Mansouri A, Adhami Mojarad MR, Badfar G, Abasian L, Rahmati S, Kooti W, et al. Epidemiology of Toxoplasma gondii among blood donors in Iran: A systematic review and meta-analysis. Transfusion and Apheresis Science, 2017; 56(3), 404-409.
Marthalia W. Chronic Toxoplasmosis Infection in Members of Cat Breeding Organization in Surabaya. Jurnal Kesehatan Lingkungan. 2020 Jan 30;12(1):48.
Wana MN, Moklas MAM, Watanabe M, Nordin N, Unyah NZ, Abdullahi SA, et al. A review on the prevalence of toxoplasma gondii in humans and animals reported in Malaysia from 2008–2018. International journal of environmental research and public health, 17(13), 4809.
Pramardika DD., Kasaluhe MD, & Sambeka Y. Studi Literatur: Analisis Faktor Risiko Toksoplasmosis Pada Wanita Indonesia. Prosiding Seminar Nasional Penelitian Poltekkes Kemenkes Tasikmalaya, 2022; 1(1),15-25.
Siransy L, Dasse SR, Dou Gonat SP, Legbedji A, N’Guessan K, Kouacou PA, et al. Immunity Status of Blood Donors Regarding Toxoplasma gondii Infection in a Low-Income District of Abidjan, Côte d’Ivoire, West Africa. J Immunol Res. 2016;2016.
Izadi M, Jafari NJ, Poornaki AM, Sadraei J, Rezavand B, Mirzaei HR, et al. Detection of Toxoplasma gondii from Clinical Specimens of Patients Receiving Renal Transplant Using ELISA and PCR. Nephro Urol Mon. 2013;5(5):983–90.
Shapiro K, Bahia-Oliveira L, Dixon B, Dumètre A, de Wit LA, VanWormer E, et al. Environmental transmission of Toxoplasma gondii: Oocysts in water, soil and food. Food and waterborne parasitology, 2019;15, e00049.
Guo M, Dubey JP, Hill D, Buchanan RL, Ray Gamble H, Jones JL, et al. Prevalence and risk factors for toxoplasma gondii infection in meat animals and meat products destined for human consumption. Journal of food protection, 2015; 78(2), 457-476.
Ekman CCJ, Chiossi MF do V, Meireles LR, de Andrade HF, Figueiredo WM, Marciano MAM, et al. Case-control study of an outbreak of acute toxoplasmosis in an industrial plant in the state of São Paulo, Brazil. Rev Inst Med Trop Sao Paulo, 2012 Sep;54(5):239–44.
Morais R dos APB, Freire ABC, Barbosa DRL, Silva L de CT da, Pinheiro AF, Costa SS da, et al. Surto de toxoplasmose aguda no Município de Ponta de Pedras, Arquipélago do Marajó, Estado do Pará, Brasil: características clínicas, laboratoriais e epidemiológicas. Rev Panamazonica Saude. 2016 Dec;7(esp):143–52.
Yılmaz A, Yazıcı E, Turk C. Assessment of seroprevalence of Toxoplasma gondii in blood donors applied to the blood center of Gazi university hospital [Internet]. Iranian Journal of Microbiology, 2021; 13(2), 243.
Hosseini SA, Golchin E, Sharif M, Sarvi S, Ahmadpour E, Rostamian A, et al. A serological investigation and genotyping of Toxoplasma gondii among Iranian blood donors indicates threat to health of blood recipients. Transfusion and Apheresis Science. 2020 Jun 1;59(3).
Afrianti D. The Incidence of Toxoplasmosis Disease on Pregnant Women Raise Cats and Not Raise Cats in Tlogosari Wetan Health Center Semarang City. Jaringan Laboratorium Medis, 2023; 5(1), 49-52.
Laksemi DAAS., Artama WT., Wijayanti MA. (2013). Seroprevalensi yang tinggi dan faktor-faktor risiko toksoplasmosis pada darah donor dan wanita di bali. Jurnal Veteriner, 2013; 14(2), 204-212.
Similar Articles
Similar Articles
- Ariel Magfirah, Diana Nur Afifah, Endang Sri Lestari, Adriyan Pramono, Ninik Rustanti, Short Chain Fatty Acid Concentrations in the Cecum of Obese Rats after Giving Dangke (Fermented Milk) from Enrekang
- Diah Navianti, Khairil Anwar, Miftahurrizqiyah, Environmental Determinants of Fecal Microplastic Abundance: A Cross-Sectional Study in the Musi River Basin, Indonesia , Poltekita: Jurnal Ilmu Kesehatan: Vol. 20 No. 1 (2026)
- Fadel Achmad Haikal, Vera Yulyani, Dhiny Easter Yanti, Analisis Faktor Penghambat Kepala Keluarga dalam Kepemilikan Jamban Keluarga , Poltekita: Jurnal Ilmu Kesehatan: Vol. 15 No. 1 (2021): May
- Adhila Fayasari, Mirta Nur Gustianti, Tri Ardianti Khasanah, Perilaku Menonton Mukbang dan Preferensi Makanan Mahasiswa di Jakarta , Poltekita: Jurnal Ilmu Kesehatan: Vol. 16 No. 2 (2022): August
- Ruliyanti Ruliyanti, Andi Wardihan Sinrang, Mardiana Ahmad, Ariyanti Saleh, Sharvianty Arifuddin, Stang Stang, The Influence of Animated Video on Knowledge and Compliance of Pregnant Women About Tetanus Toxoid Immunization , Poltekita: Jurnal Ilmu Kesehatan: Vol. 17 No. 2 (2023): August
- Prima Soultoni Akbar, Santy Irene Putri, Aris Widiyanto, Joko Tri Atmojo, Retno Dewi Prisusanti, Tarisa Aulia Ramadhanti, Telehealth Usage During The Coronavirus Disease 2019 Pandemic: A Meta-Analysis , Poltekita: Jurnal Ilmu Kesehatan: Vol. 17 No. 3 (2023): November
- Mei Ahyanti, Prayudhy Yushananta, Formulation, Dosage, and Exposure Time of Natural Substances in Controlling Aedes aegypti Larvae , Poltekita: Jurnal Ilmu Kesehatan: Vol. 17 No. 3 (2023): November
- Siti Nurunniyah, Fatimatasari, Dyah Pradnya Paramita, Claudia Banowati Subarto, Fatikha Khoirun Nisa, Asusan, Vera Tasya Ashari, Patricia Sator, Association between Maternal Characteristics and Maternal Capabilities: A Quantitative Study in The Special Region of Yogyakarta , Poltekita: Jurnal Ilmu Kesehatan: Vol. 20 No. 1 (2026)
- Haryadi, Fidyah Aminin, Ayi Diah Damayani, Dewi Mey Lestanti Mukodri, Aura Bella Gizta, Enhancing Readiness for Preeclampsia–Eclampsia among Pregnant Women and Their Husbands through Family-Based Digital Education: A Quasi-Experimental Study
- Nabila Rahma Salsa, Inge Dhamanti, Akses Pelayanan Kesehatan Ibu dan Anak di Puskesmas Selama Pandemi Covid-19 , Poltekita: Jurnal Ilmu Kesehatan: Vol. 15 No. 4 (2022): February
You may also start an advanced similarity search for this article.
Most read articles by the same author(s)
- Adita Puspitasari Swastya Putri, Mateus Sakundarno Adi, Suhartono, Nurjazuli, Egy Sunanda Putra, Analysis of Risk Factors for Anemia among Adolescent Girls in Semarang City, Central Java, Indonesia , Poltekita: Jurnal Ilmu Kesehatan: Vol. 19 No. 3 (2025)
