Amino Acid Profile of Polymeric Formula Based on Local Food for Stunting Prevention
Abstract
Adequacy energy and protein has a very strong potential to prevent stunting in children. The role of protein in preventing stunting can be focused more on the amino acid content of a food. Metabolomic research has proven that children who are stunted have lower serum concentrations of nine essential amino acids (tryptophan, isoleucine, leucine, valine, methionine, threonine, histidine, phenylalanine, lysine) compared to children who are not stunted ( p < 0.01). In addition, children who are stunted have low serum concentrations of conditionally essential amino acids (arginine, glycine, glutamine), non-essential amino acids (asparagine, glutamate, serine).This research was to ingredients the amino acid content (glutamic acid, phenylalanine, isoleucine, alanine, arginine, lysine, leucine, threonine, cystine, histidine) in polymeric formulas developed from local food. This research uses an experimental design in the laboratory with a Randomized Complete Factorial (RCF) with four factors, namely F1, F2, F3 and F4. The difference between the formulations of these four formulas is the addition of full cream milk (0%, 10%, 20% and 30% ) Analysis of the amino acid content was carried out at the SIG Laboratory Bogor. Results showed that the types of essential amino acids were complete and had a good quality amino acid score (SAA=100%). The limiting amino acid is the amino acid lysine. The digestible amino acid score in each formula is; F1 (65.72%), F2 (73.43%), F3 (81.53%), and F4 (85.25%).The results of the one-way ANOVA test showed that there were differences in amino acid content in the four formulas (P>0.05). Follow-up tests used the Tukey test to determine pairwise differences between groups and all groups, showing constant results. This shows that the treatment given, namely the addition of full cream milk, had a significant effect on the differences in amino acid content in the four formulas. Conclusion: The polymeric formula developed contains complete and good quality amino acids.
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References
Lenters, L., Wazny, K., & Bhutta ZA. Management of severe and moderate acute malnutrition in children. 2016; https://pubmed.ncbi.nlm.nih.gov/27227221/
Indonesian Ministry of Health. Regulation of the Minister of Health of the Republic of Indonesia Number 2 of 2020 concerning Child Anthropometric Standards. 2020;
Black, RE, Victora, CG, Walker, SP, Bhutta, ZA, Christian, P., De Onis, M., Ezzati, M., Grantham-McGregor, S., Katz, J., & Martorell R. Maternal and child undernutrition and overweight in low-income and middle-income countries. The Lancet. 2013; Available from: https://doi.org/https://doi.org/10.1016/S
RI K Ministry of Health. Riskendas 2018. In National Report Riskesndas 2018. 2018;
Das, JK, Salam, RA, Saeed, M., Kazmi, FA, & Bhutta ZA. Effectiveness of interventions for managing acute malnutrition in children under five years of age in low-income and middle-income countries: a systematic review and meta-analysis. 2020; https://pubmed.ncbi.nlm.nih.gov/31906272/
Hendrixson, D.T., Godbout, C., Los, A., Callaghan-Gillespie, M., Mui, M., Wegner, D., Bryant, T., Koroma, A., & Manary MJ. Treatment of severe acute malnutrition with oats or standard ready-to-use therapeutic food: a triple-blind, randomized controlled clinical trial. 2020; https://pubmed.ncbi.nlm.nih.gov/32179568/
FAO. Protein quality assessment in follow-up formula for young children and ready to use therapeutic foods. 2018;
Yi-Shen, Z., Shuai, S., & Fitz Gerald R. Mung bean proteins and peptides: Nutritional, functional and bioactive properties. Food & Nutrition Research. 2018; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5846210/
Bröer, S., & Bröer A. Amino acid homeostasis and signaling in mammalian cells and organisms. 2017;
Richard D. Semba a,⁎, Michelle Shardell b, Fayrouz A. Sakr Ashour et all. Child Stunting is Associated with Low Circulating Essential Amino Acids. EBioMedicine. 2016; https://pubmed.ncbi.nlm.nih.gov/28546457/
Michaelsen, K.F., Hoppe, C., Roos, N., Kaestel, P., Stougaard, M., Lauritzen, L., Mølgaard, C., Girma, T., & Friis H. Choice of foods and ingredients for moderately malnourished children 6 months to 5 years of age. 2009; https://pubmed.ncbi.nlm.nih.gov/19998864/
Dahiya, PK, Linnemann, AR, Van Boekel, M., Khetarpaul, N., Grewal, R.B., & Nout MJR. Technological and nutritional potential. 2015; https://pubmed.ncbi.nlm.nih.gov/24915360/
Joye I. Protein digestibility of cereal products. 2019; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6617089/
Manary, M., Callaghan, M., Singh, L., & Briend A. Protein quality and growth in malnourished children. Food and Nutrition Bulletin. 2016; https://pubmed.ncbi.nlm.nih.gov/26857118/
Braun, KVE, Erler, NS, Kiefte-de Jong, JC, Jaddoe, VW V, van den Hooven, EH, Franco, OH, & Voortman T. Dietary intake of protein in early childhood is associated with growth trajectories between 1 and 9 years of age. 2016;
Cahyono, E., & Rieuwpassa FJ. Amino acid analysis of several types of dried processed sea cucumbers in Sangihe Islands Regency. 2017; https://pubmed.ncbi.nlm.nih.gov/27733529/
Arsenault, J. E., & Brown, K. H. (2017). Effects of protein or amino-acidation on the physical growth of young children in low-income countries . Nutrition Reviews , 75 ( 9 ), 699–717. 10.1093/nutrit/nux027; https://pubmed.ncbi.nlm.nih.gov/28938793/
Arvianto, AA, Swastawati, F., & Wijayanti I. Effect of fortification of African catfish (Clarias gariepinus) meat meal on the amino acid lysine content in biscuits. Journal of Fishery Product Processing and Biotechnology. 2016; https://ejournal3.undip.ac.id/index.php/jpbhp/article/view/16019
Lima, MF de, Lima, CAR de, Dilelis, F., Gomes, AV da C., & Freitas LW de. Metabolizable energy and amino acid digestibility of soybean cake subjected to different dry extrusion temperatures for broilers. 2018; https://www.scielo.br/j/rbz/a/4RPxBJzTpYmG8NsDssPJH8y/?lang=en
Owino, VO, Irena, AH, Dibari, F., & Collins S. Development and acceptability of a novel milk‐free soybean–maize–sorghum ready‐to‐use therapeutic food (SMS‐RUTF) based on industrial extrusion cooking process. 2014; https://pubmed.ncbi.nlm.nih.gov/22462436/
Jacoeb, AM, & Lingga LAB. therapeutic food (SMS‐RUTF) based on industrial extrusion cooking process. 2012; http://onlinelibrary.wiley.com/doi/10.1111/j.1740-8709.2012.00400.x/abstract
Li, X., Zheng, S., & Wu G. Nutrition and metabolism of glutamate and glutamine in fish. Amino Acids. 2020; https://pubmed.ncbi.nlm.nih.gov/32405703/
Watford M. Glutamine and glutamate: Nonessential or essential amino acids? Animal Nutrition. 2015; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5945979/
Pedroso, JAB, Zampieri, TT, & Donato Jr J. Reviewing the effects of Lleucine supplementation in the regulation of food intake, energy balance, and glucose homeostasis. 2015; https://pubmed.ncbi.nlm.nih.gov/26007339/
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