Combination of Eucheuma Cottonii and Corticosteroids has a Superior Immunomodulatory Effect on Asthma

https://doi.org/10.33860/jik.v17i3.3247

Authors

  • Rheza Rizaldy Biomedicine Postgraduate Student, Medical Faculty of UNISSULA, Semarang City, Central Java, Indonesia
  • Sri Priantini Mulyani Department of Pediatry, Medical Faculty of UNISSULA, Semarang City, Central Java, Indonesia
  • Titiek Sumarawati Department of Chemistry, Medical Faculty of UNISSULA, Semarang City, Central Java, Indonesia

Keywords:

IL-4, IL-10, Histamine, Eucheuma cottonii, Corticosteroid

Abstract

Asthma prevalence has been increasing globally which is commonly treated using corticosteroid as gold standard treatment. However, corticosteroids have adverse effects and cases of corticosteroid-resistant asthma are emerging. Eucheuma cottonii (EC) has been shown to potentially attenuate inflammatory response in type I hypersensitivity. This study aimed to investigate the effect of Eucheuma cottonii extract and methylprednisolone combination on IL-4, IL-10, and histamine levels in Ovalbumin-induced asthma BALB/c mice. Thirty female BALB/c mice were randomly assigned to 6 groups : (1) Sham; (2) Ovalbumin-induced asthma mice; (3) Ovalbumin-induced asthma mice treated with methylprednisolone (MP) 0.24 mg/day; (4) Ovalbumin-induced asthma mice treated with EC extract 300 mg/kgBW/day; (5) Ovalbumin-induced asthma mice treated with combination of both. Treatments were given respectively for 7 days. Plasma IL-4, IL-10, and histamine levels were measured using ELISA method. The combination group showed both the lowest IL-4 levels (89.30 ± 1.37 pg/ml) and  the highest IL-10 levels (487.03 ± 20.57 pg/ml) compared to other asthma-like mouse groups (p = 0.001). No significant difference was observed in histamine levels among all treatments (p > 0.05). The administration of a combination of methylprednisolone and Eucheuma cottonii extract significantly affected plasma IL-4 and IL-10 levels compared to monotherapies. However, there was no significant difference in plasma histamine levels compared to monotherapies.

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Published

2023-11-30

How to Cite

Rizaldy, R., Mulyani, S. P., & Sumarawati, T. (2023). Combination of Eucheuma Cottonii and Corticosteroids has a Superior Immunomodulatory Effect on Asthma. Poltekita: Jurnal Ilmu Kesehatan, 17(3), 950–954. https://doi.org/10.33860/jik.v17i3.3247

Issue

Vol. 17 No. 3 (2023): November

Section

Original Articles

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Copyright (c) 2023 Rheza Rizaldy, Sri Priantini Mulyani, Titiek Sumarawati

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