Effect of Laor (Eunice viridis) Stick Supplementation on Serum Calcium and Short-Term Growth Outcomes among Undernourished Children: A Randomized Controlled Trial in Ambon City
https://doi.org/10.33860/bpk.v54i2.4431
Keywords:
Laor stick, serum calcium levels, linear growth, undernourished childrenAbstract
Background: Undernutrition remains a major public health problem among children under five years of age. Inadequate intake of protein and micronutrients contributes to poor nutritional status and growth restriction. This study aimed to analyze the effect of laor stick supplementation on serum calcium levels and short-term growth outcomes among undernourished children aged 12–59 months in Ambon City.
Methods: This single-blind, randomized trial was conducted over 60 days in Ambon City. Eligible children were assigned to groups using computer-generated random numbers with allocation concealed in opaque envelopes. Outcome assessors and laboratory analysts were blinded to the assignment. A total of 56 undernourished children completed the final analysis, consisting of 30 children in the intervention group and 26 children in the control group following 4 dropouts in the control arm. The intervention group (n = 30) received 40 g/day of laor sticks, while the control group (n = 26) received 40 g/day of tuna fish sticks serving as an active nutritional comparator. Data collection included anthropometric measurements, three-day 24-hour dietary recalls, and serum calcium examination using Atomic Absorption Spectrophotometry (AAS). Data were analyzed using paired and independent t-tests, followed by multivariate regression analysis.
Results: The intervention group showed significantly greater increases in energy, protein, and calcium intake compared to the control group (p < 0.05). Serum calcium levels increased significantly more in the intervention group compared to the control group, with a between-group mean difference of 0.24 mg/dL(95% CI: 0.17, 0.31mg/dL; p < 0.001). Significant advantages were also observed for the intervention group in body weight gain (between-group mean difference: 0.31 kg, (95% CI: 0.20, 0.42 kg; p < 0.001) and height gain (between-group mean difference: 0.42 cm (95% CI: 0.31, 0.53 cm; p < 0.001).
Conclusion: Laor stick supplementation was associated with greater short-term improvements in nutrient intake, serum calcium, body weight, and height gain compared with tuna fish sticks. Longer and larger trials using standardized growth indicators are required.
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References
Animesh Katiyar. (2022). Calcium’s role in child growth and development and it’s complications. Journal for Research in Applied Sciences and Biotechnology, 1(1), 1–6. https://doi.org/10.55544/jrasb.1.1.1
Chagwena, D. T., Fernando, S., Tavengwa, N. V., Sithole, S., Nyachowe, C., Njovo, H., Datta, K., Brown, T., Humphrey, J.H., Prendergast, A.J., Smith, L. E. (2024). Formulation and acceptability of local nutrient-dense foods for young children: A formative study for the Child Health, Agriculture and Integrated Nutrition (CHAIN) trial in rural zimbabwe. Maternal and Child Nutrition, 20(2). https://doi.org/10.1111/mcn.13605
Escobedo-monge, M. F., Parodi-román, J., Escobedo-monge, M. A., & Marugán-miguelsanz, J. M. (2025). The biological value of proteins for pediatric growth and development : A narrative review. Nutrients, 17(13), 2221. https://doi.org/10.3390/nu17132221
Gibson, R. S. (2024). Principles of nutritional assessment (3rd ed.). Oxford University Press.
Hasan, S., Naseer, S., Zamzam, M., Mohilldean, H., Van Wagoner, C., Hasan, A., Saleh, E.S., Uhley, V., Kamel-ElSayed, S. (2024). Nutrient and hormonal effects on long bone growth in healthy and obese children: A literature review. Children, 11(7), 1–16. https://doi.org/10.3390/children11070817
Hendrini, A. R., Pratiwi, R., & Setyawati, M. M. (2026). The effects of high protein supplementary feeding on the growth of stunting children aged 6-59 months in Indonesia. International Journal of Child Health and Nutrition, 15(1), 44–53. https://doi.org/10.6000/1929-4247.2026.15.01.5
Inzaghi, E., Pampanini, V., Deodati, A., & Cianfarani, S. (2022). The effects of nutrition on linear growth. Nutrients, 14(9), 1–12. https://doi.org/10.3390/nu14091752
Keats, E. C., Das, J. K., Rehana, A. A. S., Lassi, Z. S., Imdad, A., Black, R. E., & Bhutta, Z. A. (2021). Effective interventions to address maternal and child malnutrition: an update of the evidence. The Lancet Child & Adolescent Health, 5(5), 367–384. https://doi.org/10.1016/S2352-4642(20)30274-1
Kemenkes RI. (2023). Survei Kesehatan Indonesia (SKI) 2023 Dalam Angka. Kementerian Kesehatan RI
Kementerian Kesehatan RI. Peraturan Menteri Kesehatan Republik Indonesia Nomor 28 Tahun 2019 tentang angka kecukupan gizi yang dianjurkan untuk masyarakat Indonesia. Kementerian Kesehatan RI.
Kozioł-Kozakowska, A. (2023). Adequate nutrition in early childhood. Children, 10(7), 10–11. https://doi.org/10.3390/children10071155
Lestaluhu, S. R., Arifah, S. N., Lestari, U., & Lestari, S. R. (2020). Nutritional profile of kian marine worm from Kei Islands Maluku as a potential high protein food resource. Berkala Penelitian Hayati, 26(1), 17–21. https://doi.org/10.23869/bphjbr.26.1.20203
Li, R., & Cho, S. H. (2023). Substitution impact of tuna by-product meal for fish meal in the diets of rockfish (Sebastes schlegeli) on growth and feed availability. Animals, 13(22), 1–16. https://doi.org/10.3390/ani13223586
Liline, S. (2017). Analisis kadar protein cacing laor (polychaeta) dari perairan pulau Ambon. BIOPENDIX: Jurnal Biologi, Pendidikan Dan Terapan, 3(2), 167–171. https://doi.org/10.30598/biopendixvol3issue2page167-171
Mamun, A. Al, Mahmudiono, T., Yudhastuti, R., Triatmaja, N. T., & Chen, H. L. (2023). Effectiveness of food-based intervention to improve the linear growth of children under five: A systematic review and meta-analysis. Nutrients, 15(11). https://doi.org/10.3390/nu15112430
Mulyani, A. T., Khairinisa, M. A., Khatib, A., & Chaerunisaa, A. Y. (2025). Understanding stunting : impact, causes , and strategy to accelerate stunting reduction — A narrative review. Nutrients, 17(9), 1493. https://doi.org/10.3390/ nu17091493
Nurafni, Koroy, K., Muhammad, S. H., Nugroho, G. M., Kasim, S. M., & Djen, F. M. (2025). The tradition of falo laor (sea worm) as a seasonal local food products in the waters of Morotai Island. Egyptian Journal of Aquatic Biology and Fisheries, 29(5), 639–651. https://doi.org/10.21608/ejabf.2025.413283.6398
Nurhikma, Nurhayati, T., & Purwaningsih, S. (2017). Amino acid, fatty acid, and mineral content of marine worm rom South East Sulawesi. Jphpi 2017, 20(1).
Ow, M. Y. L., Tran, N. T., Berde, Y., Nguyen, T. S., Tran, V. K., Jablonka, M. J., … Huynh, D. T. T. (2025). Efficacy of long-term oral nutritional supplementation with dietary counseling on growth, body composition and bone mineralization in children with or at risk for undernutrition: a randomized controlled trial. Nutrition Journal, 24(1). https://doi.org/10.1186/s12937-025-01133-5
Parikh, P., Semba, R., Manary, M., Swaminathan, S., Udomkesmalee, E., Bos, R., … Nga, T. T. (2022). Animal source foods, rich in essential amino acids, are important for linear growth and development of young children in low- and middle-income countries. Maternal and Child Nutrition, 18(1), 1–12. https://doi.org/10.1111/mcn.13264
Rachmawati, S. N., Antika, R. B., Rohmawati, N., & Hermilasari, R. D. (2025). The formulation of edamame flour and tuna fish protein hydrolyzate biscuit as an alternative supplementary feeding for stunting. Jurnal Gizi Dan Dietetik Indonesia (Indonesian Journal of Nutrition and Dietetics), 13(1), 1. https://doi.org/10.21927/ijnd.2025.13(1).1-9
Rasheed, N. W., & Ahmad, R. I. (2021). Clinical studies about the influence of calcium intake on the biochemical parameters of bone metabolism. Journal of Physics: Conference Series, 1853(1). https://doi.org/10.1088/1742-6596/1853/1/012001
Rettob, M., Putnarubun, C., Erbabley, N., & Rahantoknam, S. (2016). Nutritional contents of lawar Perinereis cf. cultrifera (Grube 1840) from Wearlilir Waters Southeast Maluku District Indonesia. International Journal of Science and Research (IJSR), 5(9), 831–834. https://doi.org/10.21275/ART20161607
Ristanti, E. Y. (2018). Formulasi papeda instan koya laor (eunice viridis) dan efikasinya terhadap kadar hemoglobin, albumin, insulin like growth factor-1 tikus (rattus norvegicus) bunting, serta morfologi, berat badan dan panjang badan fetus. Disertasi. Universitas Airlangga.
Ristanti, E. Y., & Asrar, M. (2022). Formulation of stick laor (Eunice viridis) as an alternative food supplement for toddlers in stunting prevention. Journal of Drug Delivery and Therapeutics, 12(4), 104–111. https://doi.org/10.22270/jddt.v12i4.5593
Ristanti, E. Y., & Asrar, M. (2024). Stik laor : efektivitasnya pada pertumbuhan balita. Yogyakarta: Deepublish.
Ristanti, E. Y., Soetjipto, & Adi, A. C. (2019). Nutrition contents and sensory characteristics of the instant papeda enrich with laor (polychaeta) as supplementary food for pregnant woman. Indian Journal of Public Health Research and Development, 10(6), 416–420. https://doi.org/10.5958/0976-5506.2019.01308.1
Shertukde, S. P., Cahoon, D. S., Prado, B., Cara, K. C., & Chung, M. (2022). Calcium intake and metabolism in infants and young children: A systematic review of balance studies for supporting the development of calcium requirements. Advances in Nutrition, 13(5), 1529–1553. https://doi.org/10.1093/advances/nmac003
Siddiqa, M., Shah, G., Asif, T., Kamal, A., & Shah, B. (2026). Socioeconomic and environmental factors associated with child undernutrition and growth failure in Eastern Africa. Nutrients , 18(4), 1–22. https://doi.org/10.3390/nu18040607
Šromová, V., Sobola, D., & Kaspar, P. (2023). A brief review of bone cell function and importance. Cells, 12(21). https://doi.org/10.3390/cells12212576
Vaivada, T., Akseer, N., Akseer, S., Somaskandan, A., Stefopulos, M., & Bhutta, Z. A. (2020). Stunting in childhood : An overview of global burden, trends , determinants , and drivers of decline. The American Journal of Clinical Nutrition, 112(Suppl), 777S-791S. https://doi.org/10.1093/ajcn/nqaa159
Wairata, J., Tutupary, O. F. ., & Mangimbulu, J. C. (2020). Evaluation of fatty acid and amino acid in laor worms (Polychaetes) from tobelo, Indonesia Coastal Water. Asian Journal of Biological Sciences, 13(2), 127–133. https://doi.org/10.3923/ajbs.2020.127.133
Wati, A., & Suryana, S. (2022). Phosphorus and calcium intake of stunted toddlers aged 24-59 months: A case-control study in Sinar Bahagia Village, Simeulue. JAND: Journal of Applied Nutrition and Dietetic, 1(1), 43–48. https://doi.org/10.30867/jand.v1i1.48
Wiyono, S., Muntikah, & Meilinasari. (2023). Suplementasi makanan tambahan tinggi protein hewani, kalsium dan zinc pada anak umur 6-24 bulan sebagai upaya peningkatan panjang badan anak. Window of Health : Jurnal Kesehatan, 6(4), 354–364. https://doi.org/https://doi.org/10.33096/woh.vi.459
Xiong, T., Wu, Y., Hu, J., Xu, S., Li, Y., Kong, B., & Zhang, Z. (2023). Associations between high protein intake, linear growth, and stunting in children and adolescents: A cross-sectional study. Nutrients, 15(22), 1–15. https://doi.org/10.3390/ nu15224821
