Formulation, Dosage, and Exposure Time of Natural Substances in Controlling Aedes aegypti Larvae

https://doi.org/10.33860/jik.v17i3.3353

Authors

  • Mei Ahyanti Politeknik Kesehatan Kemenkes Tanjungkarang, Lampung, Indonesia
  • Prayudhy Yushananta Politeknik Kesehatan Kemenkes Tanjungkarang, Lampung, Indonesia

Keywords:

Aedes, Natural Substances, Effectiveness, Larvacidal, Vector

Abstract

The incidence of vector-borne diseases in Indonesia, particularly Dengue Hemorrhagic Fever (DHF), continues to escalate. This surge correlates with climate change, the extreme transition from hot to rainy seasons. The use of synthetic chemicals for control measures can also poses environmental risks; hence, there is a necessity to explore natural control methods by harnessing the biodiversity of plant species within the environment. This research aims to assess the effectiveness of botanical extract mixture on the mortality of Aedes aegypti larvae.  Method: This study utilizes an experimental design with a complete factorial random arrangement, which aimed at elucidating the effectiveness of plant extract as a bio-larvacide against Aedes aegypti mosquitoes. The effectiveness is measured through larval mortality rates based on a formulated equation, with dosage and exposure time as the research variables. The research was conducted in the Environmental Health Departement Laboratory at Health Polytechnic of Tanjungkarang from March to July 2023. The observational sheet serves as the instrument The collected data are processed and analysed using ANOVA to discern variations in larva mortality based on the formula, linear regression is applied to explore the influence of dosage and exposure time on larva mortality. Result: The result of this study exhibits that the most efficacious formulations to terminate larvaes  were determined to be the 9th, 10th, and 11th formulations.  Furthermore, an extended exposure time correlates with the escalating rate of larval demise. The statistical model prosperously accounts for 88,59% of the variability in the response pertaining to Aedes larval mortality.  Conclusion: This study discerns that individually, the formula, dosage, and exposure time, also demonstrates an impact on larval death. This study unveils that an insecticidal formula derived from soursop leaves yields a higher mortality effect compared to formulations based on other materials.

References

Naish S, Dale P, Mackenzie JS, McBride J, Mengersen K, Tong S. Climate change and dengue: a critical and systematic review of quantitative modelling approaches. BMC infectious diseases. 2014 Dec;14(1):1-4. https://doi.org/10.1186/1471-2334-14-167

Gubler DJ. The economic burden of dengue. The American journal of tropical medicine and hygiene. 2012 May 5;86(5):743. doi:10.4269/ajtmh.2012.12-0157

Halasa YA, Shepard DS, Zeng W. Economic cost of dengue in Puerto Rico. Am J Trop Med Hyg. 2012;86(5):745-752. https://doi.org/10.4269/ajtmh.2012.12-0157

Kemenkes RI. Demam Berdarah Dengue. Bul Jendela Epidemiol. 2010;2:48.

Mufarida B. Kemenkes Catat 84.734 Kasus DBD, Sebaran Tertinggi Ada di Pulau Jawa. Okenews.

Yushananta P. Dengue Hemorrhagic Fever and Its Correlation with The Weather Factor In Bandar Lampung City: Study From 2009-2018. J Aisyah J Ilmu Kesehat. 2021;6(1):117-126.

Fathonah A. Uji Toksisitas Ektrak Daun Dan Biji Cariya Papaya Sebagai Larvasida Anopheles Aconitus. Program Studi Biologi Fakultas Sain dan Teknologi Universitas Islam Negeri Sunan Kalijaga Yogyakarta; 2013.

Murdani R. Keefektifan Daya Bunuh Ekstrak Daun Jeruk Nipis (Citrus Aurantifolia) Terhadap Larva Nyamuk Aedes Aegypti Instar III. Program Studi Kesehatan Masyarakat Ilmu Kesehatan Unversitas Muhammadiyah Surakarta; 2014.

Yuniar N, Majid R, Ode L, Zety M, Wildan E. Larvacidal effect of papaya leaf extracts (Carica papaya l.) toward the larvae of Anopheles aconitus donits mosquitoes as an effort to prevent malaria disease in Rural Areas of Southern Konawe. Proceeding Int Semin Glob Heal 2017. 2017;1(1):333-343.

Purnama SG. Diktat Pengendalian Vektor. Prodi IKM FK Universitas Udayana. 2017:4-50.

Ahdiyah I, Purwani KI. Pengaruh ekstrak daun mangkokan (Nothoponax scutellarium) sebagai larvasida Nyamuk culex sp. Sain dan Seni ITS. 2015;3(1).

Ramayanti I, Febriani R. Uji Efektivitas Larvasida Ekstrak Daun Pepaya ( Carica papaya Linn ) terhadap Larva Aedes aegypti Pendahuluan Nyamuk yang ada . Spesies ini dapat ditemukan aegypti di Indonesia . Bisa dikatakan sebagai yang telah resisten , salah satunya Metode Penelitian. Syifa’MEDIKA. 2016;6(2):79-88.

Yushananta P, Mei. The Effectiveness of Betle Leaf (Piper betle L.) Extract as a Bio- pesticide for Controlled of Houseflies (Musca domestica L.). Open Access Maced J Med Sci. 2021;9(E):895-900. https://doi.org/10.3889/oamjms.2021.6886

Adibah A, Dharmana E. Larvicidal Effectiveness Test of Boiled Betle Leaf (Piper betle L.) Against Aedes aegypti Larvae : Studies in Value of Lc50, Lt50, and The Rate of Larvae Mortality. Diponegoro Med J. 2017;6(2):244-252.

Anisah, Sukesi T. Uji Efektifitas Ekstrak Daun Sirih ( Piper betle L ) S ebagai Larvasida Larva Lalat Rumah ( Musca domestica ) Effectiveness of Sirih Leaf Extract ( Piper b etle L ) as A House Fly Larvae ( Musca Domestica ) Larvicidal. J Vektor Penyakit. 2018;12(1):39-46. https://doi.org/10.22435/vektorp.v12i1.283

Praulins G, McDermott DP, Spiers A, Lees RS. Reviewing the WHO tube bioassay methodology: Accurate method reporting and numbers of mosquitoes are key to producing robust results. Insects. 2022 Jun 14;13(6):544. https://doi.org/10.3390/insects13060544

Sayono, Nurullita U. Situasi Terkini Vektor Dengue (Aedes Aegypti) Di Jawa Tengah. KEMAS J Kesehat Masy. 2016;11(2):96-105.

Efriadi H, Mutiara D, Emilia I. Uji Toksisitas Akar Tuba (Derris eliptica) terhadap Mortalitas Benih Ikan Nila (Oreochromis sp). Sainmatika J Ilm Mat dan Ilmu Pengetah Alam. 2018;15(1):56. doi:10.31851/sainmatika.v15i1.1783

WHO. Guidelines for Laboratory and Field Testing of Mosquito Larvicides. World Health Organization Communicable Disease Control, Prevention and Eradication, WHO Pesticide Evaluation Scheme; 2005. http://whqlibdoc.who.int/hq/2005/WHO_CDS_WHOPES_GCDPP_2005.13.pdf?ua=1

Ahyanti M, Yushananta P. Kombinasi Ekstrak Daun Tapak Dara (Catharanthus roseus) Dan Daun Sirsak (Annona muricata) Sebagai Bio-larvasida. Ruwa Jurai J Kesehat Lingkung. 2022;16(3):113. doi:10.26630/rj.v16i3.3611

Melliska CE. Efektivitas Larvasida Ekstrak Daun Sirsak ( Annnona Muricata Lina) Terhadap Kematian Jentik Culex Sp (Studi Kasus di-Gampong’’Purwodadi, Kecamatan: Kuala*Pesisir,_Kabupaten&Nagan!Raya). PREPOTIF J Kesehat Masy. 2022;6(2):1782-1786.

Syazana N. Kajian Literatur Efektivitas Biolarvasida Ekstrak Daun Sirsak Terhadap Jentik Nyamuk Aedes Aegypti. Environ Occup Heal Saf J •. 2022;2(2):133.

Ahyanti M, Yushananta P. Kandungan Saponin dan Flavonoid Pada Tanaman Pekarangan serta Potensinya Sebagai bioinsektisida Lalat Rumah (Musca domestica). Ruwa Jurai J Kesehat Lingkung. 2023;17(1):31-43. http://dx.doi.org/10.26630/rj.v17i1.3763

Saputri AE, Hariyanti DB, Ramadhani IA, Harijani WS. Potensi Daun Lamtoro (Leucaena leucocephala) Sebagai Biopestisida Ulat Grayak (Spodoptera litura F.). Agritrop J Ilmu-Ilmu Pertan (Journal Agric Sci. 2021;18(2):209-216. doi:10.32528/agritrop.v18i2.3740

Jannah NAM, Yuliani Y. Keefektifan Ekstrak Daun Pluchea indica dan Chromolaena odorata sebagai Bioinsektisida Terhadap Mortalitas Larva Plutella xylostella. LenteraBio Berk Ilm Biol. 2021;10(1):33-39.

Laksono FW, Sari NLS, Salsabila S, Kurniasari L. Pengaruh Insektisida Alami Ekstrak Daun Jelatang (Urtica Dioica L.) Terhadap Mortalitas Larva Aedes Aegypti. Pros Sains Nas dan Teknol. 2022;12(1):1-8.

Sitohang M, Mamahit JME, Pakasi SE. Inovasi bomb fizzies antifeedant dari ekstrak daun pangi (Pangium edule Reinw.) untuk pengendalian hama kubis Plutella xylostella L. J Agroteknologi Terap. 2022;3(2):124-130.

Asfahani F, Halimatussakdiah, Amna U. Analisis Fitokimia Ekstrak Daun Sirsak ( Annona muricata Linn .) dari Kota Langsa. Quim J Kim Sains dan Terap. 2022;4(2):18-22.

Kusumawati WD, Subagiyo A, Firdaust M. Pengaruh Beberapa Dosis dan Jenis Ekstrak Larvasida Alami Terhadap Kematian Larva Nyamuk Aedes aegypti. Bul Kesehat Lingkung Masy. 2018;37(3):283-295.

Fajriyati AF, Anwar S, Kusmiyati F. Aplikasi ekstrak daun tapak dara (Catharanthus roseus l.) terhadap pertumbuhan dan morfologi tanaman bawang daun (Allium fistulotum l.). J Ilm Pertan. 2022;19(1):29-37.

Andriani T, Fikri EN, budi IS. Uji Efektifitas Serbuk Tanaman Tapak Dara Uji Efektivitas Serbuk Tanaman Tapak Dara (Catharanthus roseus L.) Terhadap Serangan Nematoda Puru Akar Meloidogyne spp. pada Tomat (Lycopersicum esculentum Mill.). Prot Tanam Trop. 2018;1(2):36-39.

Dewi IALP, Damriyasa IM, Dada IKA. Bioaktivitas Ekstrak Daun Tapak Dara (Catharanthus Roseus) Terhadap Periode Epitelisasi Dalam Proses Penyembuhan Luka Pada Tikus Wistar. Indones Med Veterinus. 2013;2(1):58-75.

Sayono, U N, M S. Pengaruh konsentrasi flavonoid dalam ekstrak akar tuba (Derris eliptica) terhadap kematian larva Aedes aegypti. Kesehat Masy Indones. 2010;6(1):38-47.

Fauzana H, Faradilla N. Uji Konsentrasi Ekstrak Daun Krinyuh (Eupatorium odoratum L.) sebagai Racun Perut terhadap Mortalitas Ulat Grayak (Spodoptera litura F.). Agroteknologi Trop. 2018;7(2):108-115.

Maheswari PP, Wijaya IN, Sritaman M. Uji Efektivitas Beberapa Jenis Ekstrak Daun Tanaman Terhadap Perkembangan Ulat Daun Kubis (Plutella xylostella L.) di Laboratorium. E-Jurnal Agroekoteknologi Trop. 2018;7(3):392-399. Retrieved from https://ojs.unud.ac.id/index.php/JAT392

Callier V, Nijhout HF. Control of body size by oxygen supply reveals size-dependent and size-independent mechanisms of molting and metamorphosis. Proc Natl Acad Sci U S A. 2011;108(35):14664-14669.

Ahyanti M, Yushananta P, Usman S. Efektifitas Beberapa Tanaman dalam Mengendalikan Lalat Rumah (Musca domestica). Poltekita: Jurnal Ilmu Kesehatan. 2022 Feb 21;15(4):398-402.

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Published

2023-11-19

How to Cite

Ahyanti, M., & Yushananta, P. (2023). Formulation, Dosage, and Exposure Time of Natural Substances in Controlling Aedes aegypti Larvae . Poltekita: Jurnal Ilmu Kesehatan, 17(3), 783–791. https://doi.org/10.33860/jik.v17i3.3353

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